/* * calibrate-detector.c * * Detector calibration * * (c) 2006-2010 Thomas White * * Part of CrystFEL - crystallography with a FEL * */ #ifdef HAVE_CONFIG_H #include #endif #include #include #include #include #include #include #include #include "utils.h" #include "hdf5-file.h" #include "filters.h" #include "peaks.h" #include "thread-pool.h" #define INTEGRATION_RADIUS (10) typedef enum { SUM_THRESHOLD, SUM_PEAKS } SumMethod; struct sum_args { char *filename; int config_cmfilter; int config_noisefilter; double *sum; int w; int h; SumMethod sum_method; double threshold; }; struct queue_args { FILE *fh; char *prefix; int config_cmfilter; int config_noisefilter; double *sum; int w; int h; SumMethod sum_method; double threshold; }; static void show_help(const char *s) { printf("Syntax: %s [options]\n\n", s); printf( "Calibrate detector geometry from FEL diffraction images.\n" "\n" " -h, --help Display this help message.\n" "\n" " -i, --input= Specify file containing list of images to process.\n" " '-' means stdin, which is the default.\n" " -o, --output= Output filename for summed image in HDF5 format.\n" " Default: summed.h5.\n" "\n" " -p, --intermediate=

Stem of filename for intermediate images.\n" " The filename stem

will be postfixed with a\n" " hyphen, the current number of patterns processed\n" " and '.h5'. Such a pattern will be saved after\n" " every 1000 input patterns.\n" " If this option is not specified, no intermediate\n" " patterns will be saved.\n" "\n" " -s, --sum= Use this method for summation. Choose from:\n" " peaks : sum 10px radius circles around peaks.\n" " threshold : sum thresholded images.\n" " -t, --threshold= Set the threshold if summing using the 'threshold'\n" " method.\n" "\n" " --filter-cm Perform common-mode noise subtraction on images\n" " before proceeding.\n" " --filter-noise Apply an aggressive noise filter which sets all\n" " pixels in each 3x3 region to zero if any of them\n" " have negative values.\n" "\n" " -j Run analyses in parallel. Default 1.\n" " -x, --prefix=

Prefix filenames from input file with 'p'.\n"); } static void sum_peaks(struct image *image, double *sum) { int x, y, i; int w = image->width; const int lim = INTEGRATION_RADIUS * INTEGRATION_RADIUS; /* FIXME: Get threshold value from command line */ search_peaks(image, 800.0, 100000.0); for ( i=0; ifeatures); i++ ) { struct imagefeature *f = image_get_feature(image->features, i); int xp, yp; /* This is not an error. */ if ( f == NULL ) continue; xp = f->x; yp = f->y; for ( x=-INTEGRATION_RADIUS; x<+INTEGRATION_RADIUS; x++ ) { for ( y=-INTEGRATION_RADIUS; y<+INTEGRATION_RADIUS; y++ ) { /* Circular mask */ if ( x*x + y*y > lim ) continue; if ( ((x+xp)>=image->width) || ((x+xp)<0) ) continue; if ( ((y+yp)>=image->height) || ((y+yp)<0) ) continue; float val = image->data[(x+xp)+w*(y+yp)]; sum[(x+xp)+w*(y+yp)] += val; } } } } static void sum_threshold(struct image *image, double *sum, double threshold) { int x, y; for ( x=0; xwidth; x++ ) { for ( y=0; yheight; y++ ) { float val = image->data[x+image->width*y]; if ( val > threshold ) { sum[x+image->width*y] += val; } } } } static void add_image(void *args, int cookie) { struct sum_args *pargs = args; struct hdfile *hdfile; struct image image; image.features = NULL; image.data = NULL; image.flags = NULL; image.indexed_cell = NULL; image.filename = pargs->filename; image.cpeaks = NULL; image.n_cpeaks = 0; image.det = NULL; STATUS("%3i: Processing '%s'\n", cookie, pargs->filename); hdfile = hdfile_open(pargs->filename); if ( hdfile == NULL ) { return; } else if ( hdfile_set_first_image(hdfile, "/") ) { ERROR("Couldn't select path\n"); hdfile_close(hdfile); return; } /* FIXME: Nominal photon energy */ hdf5_read(hdfile, &image, 1, 2000.0); if ( pargs->config_cmfilter ) { filter_cm(&image); } if ( pargs->config_noisefilter ) { filter_noise(&image, NULL); } if ( (pargs->w != image.width) || (pargs->h != image.height) ) { ERROR("Wrong image size.\n"); goto out; } switch ( pargs->sum_method ) { case SUM_THRESHOLD : sum_threshold(&image, pargs->sum, pargs->threshold); break; case SUM_PEAKS : sum_peaks(&image, pargs->sum); break; } out: free(image.data); image_feature_list_free(image.features); if ( image.flags != NULL ) free(image.flags); hdfile_close(hdfile); free(pargs->filename); free(pargs); } static void *get_image(void *qp) { char line[1024]; struct sum_args *pargs; char *rval; struct queue_args *qargs = qp; /* Get the next filename */ rval = fgets(line, 1023, qargs->fh); if ( rval == NULL ) return NULL; pargs = malloc(sizeof(struct sum_args)); pargs->w = qargs->w; pargs->h = qargs->h; pargs->sum_method = qargs->sum_method; pargs->threshold = qargs->threshold; pargs->config_cmfilter = qargs->config_cmfilter; pargs->config_noisefilter = qargs->config_noisefilter; pargs->sum = qargs->sum; chomp(line); pargs->filename = malloc(strlen(qargs->prefix) + strlen(line) + 1); snprintf(pargs->filename, 1023, "%s%s", qargs->prefix, line); return pargs; } int main(int argc, char *argv[]) { int c; char *filename = NULL; char *outfile = NULL; FILE *fh; int n_images = 0; int config_cmfilter = 0; int config_noisefilter = 0; char *prefix = NULL; char *sum_str = NULL; char *intermediate = NULL; double threshold = 400.0; SumMethod sum; int nthreads = 1; struct queue_args qargs; int n_done; const int chunk_size = 1000; /* Long options */ const struct option longopts[] = { {"help", 0, NULL, 'h'}, {"input", 1, NULL, 'i'}, {"output", 1, NULL, 'o'}, {"filter-cm", 0, &config_cmfilter, 1}, {"filter-noise", 0, &config_noisefilter, 1}, {"prefix", 1, NULL, 'x'}, {"sum", 1, NULL, 's'}, {"intermediate", 1, NULL, 'p'}, {"threshold", 1, NULL, 't'}, {0, 0, NULL, 0} }; /* Short options */ while ((c = getopt_long(argc, argv, "hi:x:j:o:s:p:t:", longopts, NULL)) != -1) { switch (c) { case 'h' : show_help(argv[0]); return 0; case 'i' : filename = strdup(optarg); break; case 'o' : outfile = strdup(optarg); break; case 'x' : prefix = strdup(optarg); break; case 'j' : nthreads = atoi(optarg); break; case 's' : sum_str = strdup(optarg); break; case 'p' : intermediate = strdup(optarg); break; case 't' : threshold = atof(optarg); break; case 0 : break; default : return 1; } } if ( filename == NULL ) { filename = strdup("-"); } if ( strcmp(filename, "-") == 0 ) { fh = stdin; } else { fh = fopen(filename, "r"); } if ( fh == NULL ) { ERROR("Failed to open input file '%s'\n", filename); return 1; } free(filename); if ( sum_str == NULL ) { STATUS("You didn't specify a summation method, so I'm using" " the 'peaks' method, which gives the best results.\n"); sum = SUM_PEAKS; } else if ( strcmp(sum_str, "peaks") == 0 ) { sum = SUM_PEAKS; } else if ( strcmp(sum_str, "threshold") == 0) { sum = SUM_THRESHOLD; } else { ERROR("Unrecognised summation method '%s'\n", sum_str); return 1; } free(sum_str); if ( prefix == NULL ) { prefix = strdup(""); } if ( outfile == NULL ) { outfile = strdup("summed.h5"); } if ( nthreads == 0 ) { ERROR("Invalid number of threads.\n"); return 1; } qargs.w = 1024; /* FIXME! */ qargs.h = 1024; /* FIXME! */ qargs.sum_method = sum; qargs.threshold = threshold; qargs.config_cmfilter = config_cmfilter; qargs.config_noisefilter = config_noisefilter; qargs.sum = calloc(qargs.w*qargs.h, sizeof(double)); qargs.prefix = prefix; qargs.fh = fh; do { n_done = run_threads(nthreads, add_image, get_image, (void *)&qargs, NULL, chunk_size); n_images += n_done; /* Write intermediate sum if requested */ if ( (intermediate != NULL) && (n_done == chunk_size) ) { char outfile[1024]; snprintf(outfile, 1023, "%s-%i.h5", intermediate, n_images); hdf5_write(outfile, qargs.sum, qargs.w, qargs.h, H5T_NATIVE_DOUBLE); } } while ( n_done == chunk_size ); /* Write the final output */ hdf5_write(outfile, qargs.sum, qargs.w, qargs.h, H5T_NATIVE_DOUBLE); free(qargs.sum); free(prefix); free(outfile); if ( intermediate != NULL ) free(intermediate); fclose(fh); STATUS("There were %i images.\n", n_images); return 0; }